The recent advent of genetic tools, such as Peturb-seq, that combine the analysis of pooled CRISPR screens with single-cell RNA sequencing has delivered unprecedented insights into mammalian gene function and genetic regulatory networks. However, current implementations of these tools face technical and practical limitations that restrict their use. To overcome these challenges, researchers at University of California - San Francisco, Princeton University, and 10x Genomics have developed “direct-capture” Peturb-seq, which enables substantially higher throughput investigations into genetic interactions. Furthermore, through the addition of a hybridization-based target enrichment methodology, sensitive and specific sequencing of biologically meaningful panels of transcripts is now possible — significantly reducing experimental costs. In this webinar you will… Learn how Perturb-seq, which combines pooled CRISPR screens with single-cell RNA-sequencing, facilitates unbiased exploration of gene function and delineation of genetic regulatory networks. Hear about the recent innovation of direct-capture Perturb-seq, which significantly increases screening throughput – substantially expanding the scale and flexibility of this technology. Understand how targeting individual genes with multiple sgRNAs per cell improves efficacy of CRISPR interference and activation, facilitating the use of compact, highly active CRISPR libraries for single-cell screens. See how hybridization-based target enrichment can decrease sequencing costs for transcripts of interest by up to 90%. Discover how the scalability and accuracy of Twist’s CRISPR Oligo Pools and NGS Target Enrichment solutions supported the development and application of this ground-breaking technology.

In the post-genome era, large-scale, gene-centric target discovery studies using tools such as RNAi and CRISPR have proliferated, and produced many insights into disease biology. Challenges remain however, as these tools often struggle to interrogate the subtle biological processes that govern clinical response. Fortunately, new screening tools exist that can both find targets and uncover the mechanisms that modulate complex molecular interactions. One such tool is SITESEEKER®, developed by the scientists at PhoreMost.  In this webinar you will: Hear how SITESEEKER® exploits protein interference (PROTEINi) to deliver a substantially greater magnitude of complexity than existing screening tools. See how the path from target ID to drug development can be shortened using PROTEINi, through the discovery of druggable pockets in addition to targets. Discover how this technology can be applied in key disease areas, including in cancer biology, neurodegeneration and for hijacking targeted protein degradation. Learn why the length and fidelity of Twist Oligo Pools is enabling PhoreMost to develop the next generation of their platform.

Functional genetic screening using the CRISPR/Cas9 system is a powerful tool for interrogating gene function at scale, but access to and implementation of this technology is constrained by existing pre-defined CRISPR sgRNA libraries. Here, a single Twist Oligo Pool was used in combination with Custom Library Multiplexed Cloning (CLUE; crispr-clue.de), an easy-to-use bioinformatics and wet-lab workflow, to generate numerous high-quality, custom sgRNA libraries. The exceptional fidelity of Twist Oligo Pools preserves the uniform representation of sgRNAs in the resulting CLUE libraries without cross-library contamination, meeting the stringent requirements for success in CRISPR screening applications.