The simple, fast, most cost-effective way to get linear dsDNA. Our industry-leading synthesis quality means less colony screening to find the perfect clone.
Twist’s DNA synthesis technology outperforms the competition with exceptionally low error rates.
In this study, Twist Gene Fragments have an average of 2-fold greater accuracy in sequence fidelity for Twist Gene Fragments over IDT eBlocks™ and gBlocks™ Gene Fragments.
Get a higher percentage of perfect clones with Twist Gene Fragments, reducing your time screening colonies.
In this study of 63 unique sequences, our low error rate meant Twist Gene Fragments consistently yielded the highest percentage of perfect clones when compared to IDT eBlocks™ and gBlocks™ Gene Fragments. Cloning efficiency is a crucial factor for determining sequencing costs, which can add up when screening colonies.
Colony picking calculator
Our industry-leading fragment synthesis error rate means you need to pick fewer colonies to find the perfect clone. Estimate how many colonies you need to pick here*.
*Colony numbers vary depending on sequence complexity, purity, toxicity, and cloning method. In some cases, picking 2–3 times more colonies than estimated here may be necessary.
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Product specifications
| Specification | Value | Details |
|---|---|---|
| Product format | Linear dsDNA | |
| Length | 0.3 kb – 5.0 kb | |
| Complexity | Low | Medium |
A sequence's complexity score is based on the combined presence, frequency, and length of various features within the design of a sequence. Impacts the ease and speed of production. High complexity sequences are available as Clonal Genes. |
| Codon optimization | Large language model-based | Available for protein expression in hundreds of target organisms. |
| Amplification adapters Add-on |
|
Available to be appended to the 5' and 3' ends of all sequences, allowing for single primer set amplification. 5’– CAATCCGCCCTCACTACAACCG-<insert sequence>-CTACTCTGGCGTCGATGAGGGA – 3’ |
| Specification | Value | Details |
|---|---|---|
| Turnaround time | 2 – 4 business days* | |
| Yield | 100 ng – 1 µg | |
| Container | 2 mL tube, 96-well plate or 384-well plate |
|
| Concentration normalization Add-on | 250 ng/fragment | |
| Delivery format Add-on | Dried down or resuspended |
|
| Shipment conditions | Ambient or frozen on dry ice |
|
Every Gene Fragment we ship includes a Certificate of Analysis (COA) that contains the following information.
| Specification | Value | Details |
|---|---|---|
| Yield measurement | Fluorometric quantification | |
| Length verification | Capilliary electrophoresis |
Customers often pair Twist Genes with the following products
Partner with our team of experts to navigate the entire discovery funnel from target to lead.
High-diversity, silicon-synthesized oligo pools designed for high-throughput screening and complex library construction.
Explore vast sequence space with high-diversity libraries where every variant is base-by-base synthesized and NGS-verified to match your design.
Twist’s Technical Support team is available to help you tackle your biggest research challenges. If you have any questions about your project, our scientists are here to find the answer.
For Research Use Only.
Not for Diagnostic Procedures.
*This timeframe refers to the typical processing and handling time within our facilities before your order is handed over the shipping carrier. Gene Fragments are shipped in 2-4 business days. Orders placed outside of the US will incur additional delivery time.
†This is for illustrative purposes and the stated benefits, support, benefit calculations and other results are estimates only, based on input from a limited number of existing Twist customers and various assumptions made by the potential customers. This estimate does not constitute a proposal or quote.
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681 Gateway Blvd
South San Francisco, CA 94080
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