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Complex features we support

Our Gene Synthesis platform helps you make difficult sequences in predictable timescales. See
which complex features we can support below.

 

Short tandem repeats up to 100 bp

Regions of 3-9 bp that repeat multiple times, with up to 100% homology between repeats.

 

Long repeats up to 200 bp

Regions ≥ 10 bp with up to 100% homology that repeat more than once within a single sequence.
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Inverted repeats (hairpins) up to 100 bp

Repeats complementary to each other, with up to 100% homology between repeats.

 

Localized GC content between 25–75%

GC content within a 50 bp window.

 

Homopolymers up to 30 bp

Runs of an identical base.

 

Average GC content between 10–90%

Average GC content across the whole sequence.
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Product details

Complex DNA. Simple pricing.

With prices starting at 12¢ per base, you get more complex DNA for your budget. Cost-per-base scales with sequence length, not sequence complexity, so pricing stays predictable. And, with early access, you get an immediate quote for your Complex Genes in our ordering portal.

See pricing

US manufacturing

All Gene products are USA-made, shipping globally from our factory in Wilsonville, OR. Here, our scientists use ultra-long oligo synthesis on silicon, automated enzymatic assembly, and long-read sequencing to produce your Complex Gene Synthesis projects.

Reliable turnaround

For Complex Genes, we provide an estimated turnaround time before you order as well as live manufacturing tracking on our portal. We aim to complete all Complex Gene orders within 15 business days. We will contact you immediately should challenges arise.

Support from real scientists

Twist’s Technical and Customer Support teams are available to help you tackle your biggest research challenges. If you ever have a question or issue, click on the chat icon in the bottom-right of our website, and connect with a real scientist in minutes.

*For Research Use Only. Not for Diagnostic Procedures.

The prices listed apply exclusively to orders placed within our direct service countries. For regions serviced by our authorized distributors, pricing, taxes, and shipping fees may vary. Customers located in distributor-managed territories are encouraged to contact their local representative directly for an accurate quote and availability.

*For Research Use Only. Not for Diagnostic Procedures.

*We will aim to ship all complex genes within 15 business days.

*For Research Use Only. Not for Diagnostic Procedures.

Product data

Complex DNA. Simple pricing.

Manage projects with predictable costs. While Complex Genes cost a little more than standard gene synthesis, we don't change our cost-per-base on a per-sequence basis. Get an immediate quote for your Complex Genes order in our ordering portal.

See pricing

*For Research Use Only. Not for Diagnostic Procedures.

The prices listed are in USD, and apply exclusively to orders placed within our direct service countries. For regions serviced by our authorized distributors, pricing, taxes, and shipping fees may vary. Customers located in distributor-managed territories are encouraged to contact their local representative directly for an accurate quote and availability.

US manufacturing

All Gene products are USA-made, shipping globally from our factory in Wilsonville, OR. Here, our scientists use ultra-long oligo synthesis on silicon, automated enzymatic assembly, and long-read sequencing to produce your Complex Gene Synthesis projects.

*For Research Use Only. Not for Diagnostic Procedures.

Reliable turnaround

For Complex Genes, we will provide estimated turnaround time before you order as well as live manufacturing tracking on our portal. We will aim to complete all Complex Gene orders within 15 business days. We will contact you immediately should challenges arise.

*We will aim to ship all complex genes within 15 business days.

Support from real scientists

Twist's Technical Support team is available to help you tackle your biggest research challenges. If you ever have a question or issue, click on the chat icon in the bottom-right of our website, and connect with a real scientist in minutes.

*For Research Use Only. Not for Diagnostic Procedures.

"A key part of our work with external partners is keeping to our agreed project delivery timelines. Having a reliable synthesis partner in Twist is a huge factor in the success of this work, as they make it easy to track the progress of my order, deliver genes on time, and actively communicate whenever there is an issue."

Imogen Binnan, PhD
Lead Biologist, Resurrect Bio

*Results are specific to the institution where they were obtained and may not reflect the results achievable at other institutions

"The online ordering experience on Twist is by far the smoothest and most delightful of all providers I have tried. Together with the competitive pricing, Twist is our team's go-to supplier for DNA Fragments, Clonal Genes, and Oligo Pools."

David Lips
Frontier Development, Spiber Inc.

*Results are specific to the institution where they were obtained and may not reflect the results achievable at other institutions

"I am a structural biologist working on proteins with diverse functions from a wide range of organisms. Our research requires extensive gene screening, and therefore gene synthesis must be accurate, fast, and cost-effective. Genes synthesized by Twist Bioscience have consistently shown a low error rate and reliable quality, while being delivered rapidly and at a reasonable cost. We look forward to continuing to work with Twist Bioscience in the future."

Masahiro Fukuda, Ph.D.
Assistant Professor, The Research Center for Advanced Science and Technology (RCAST)

*Results are specific to the institution where they were obtained and may not reflect the results achievable at other institutions

"Twist Bioscience saved me during my PhD! I ordered countless synthetic genes from them. Whenever a cloning procedure didn’t work in lab, they provided the entire plasmid, which made a huge difference. I even used their services to build our own nanobody library. Their reliable and high-quality products were essential to the success of my research!!"

Claudia D'Ercole
Postdoctoral researcher, Laboratory for Environmental and Life Sciences, Univerza v Novi Gorici, Slovenija

*Results are specific to the institution where they were obtained and may not reflect the results achievable at other institutions

How to get simple, transparent ordering
for Complex Genes

Try now
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Upload and review your sequences

We analyze your sequences for manufacturability. Modify your sequences if needed. Immediate pricing and turnaround estimates are provided.

How it works
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Customize your order

Choose your prep scale, endotoxin level, required quality documentation, normalization and delivery format.

How it works step 2
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Checkout and receive your Complex Genes

Generate a quote and check out. Track your order in real time as it is manufactured. You’ll hear from one of our DNA experts immediately should an issue arise.

How it works step 3

FAQ

Can I order all of my sequences as Express Genes?
Clonal Gene sequences scored as Low or Medium complexity are eligible for the Express service. The Twist Bioscience eCommerce platform will score all sequences once they are uploaded and flag which ones are eligible for the Express service.
What are the sequence acceptance criteria for Express Genes?
We use a three-tier scoring system to determine the manufacturability and turnaround time of your genes:

Low Complexity: Standard designs with predictable manufacturing and our fastest turnaround.
Medium Complexity: Designs that require additional manufacturing precautions but do not require changes to your sequence.
High Complexity: Highly intricate designs requiring specialized workflows and extended turnaround times.

Modify: This status appears if your sequence falls outside our complexity parameters, is too long/short, or contains non-standard characters.


Our algorithm flags sequences as High Complexity if they meet any of the following criteria:

GC Content: Local GC content within a 50 bp window falls below 10% or exceeds 90%.
Repeats: Direct repeats >200 bp; Short Tandem Repeats (STRs) >100 bp.
Secondary Structures:Hairpin-forming repeats >100 bp.
Homopolymers:Any single-nucleotide run (A, T, G, or C) >30 bp.
Length: Any sequence between 300 bp and 7000 bp that triggers the above flags.

Note: For Medium and High complexity sequences, our website will now display specific warning flags to show you exactly which features are driving the complexity score.
What are the differences between the Express and Standard Clonal Gene offerings?
The Express service is an upgraded service for Clonal Genes that provides a faster turnaround time than our Standard service.

Express: 4-7 business days turnaround; DNA Scales: 1 μg - 4 μg, 4 μg - 10 μg, 10 μg - 100 μg, 100 μg - 1 mg (turnaround for 10 μg - 100 μg and 100 μg - 1 mg is 7-10 business days); Complexity: Low and Medium only; Size: 0.3 - 7.0 kb; Vectors: Catalog and Custom; Antibiotics: AMP, KAN, CHL.

Standard: 10 business days turnaround; Same DNA Scales; Complexity: High, Medium or Low ; Size: 0.3 - 7.0 kb; Vectors: Catalog and Custom; Antibiotics: AMP, KAN, BLS, CHL, SPE, TET, ZEO.
What does it mean if the Express icon is grayed out for a sequence?
Only Low and Medium complexity sequences are eligible for the Express service. If a gene sequence is grayed out under the Express Genes section, that means it is a High complexity or needs to be modified (score appears as "Modify") sequence and will be marked as such in the eCommerce platform.
How do I upload my sequences to the Twist platform?
Our updated uploader is designed for maximum flexibility. You can upload an Excel template or a CSV file containing your sequence names and the sequences themselves.

Auto-Detection: The algorithm automatically detects if your sequences are in DNA or Amino Acid format.
Flexible Mapping: The system automatically identifies columns for Well, Plate, and Custom Plate Maps.
Post-Upload Editing: If your file doesn't contain location data, don't worry—you can specify the Well and Plate information directly in the interface after the file has been uploaded.
Will my codon-optimized sequence be manufacturable?
We have decoupled manufacturability parameters from the core optimization to focus primarily on protein expression. However, if the algorithm cannot find a manufacturable solution, it will automatically attempt to fix localized regions of extreme complexity. We do this while minimizing any impact on the expression profile.
Important: While we optimize for the best possible DNA composition, please remember that protein expression is multifactorial; DNA sequence is just one of many variables influencing your final yield.
Have additional questions? Check out our FAQ page

Our Twist scientists are here to help.

 
Get in touch

*For Research Use Only. Not for Diagnostic Procedures.

Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080