The Twist Library Preparation Enzymatic Fragmentation (EF) Kit 2.0 is built to help you achieve a more efficient sequencing pipeline and get more accurate Next-Generation Sequencing (NGS) results. Compared to the original Twist Library Preparation EF Kit 1.0, this updated kit has several benefits. In addition to new fragmentation and ligation enzymes, the kit now includes the Equinox Library Prep Amp Mix. This hot-start enzyme formulation has an improved error rate* and high efficiency at low input volumes—making it ideal for samples such as liquid biopsies, where high efficiency and yield are necessary.